Invitrogen™ CENPB Monoclonal Antibody (A5F5)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA542558

Catalog No. PIMA542558

Description

Positive Control: Hela cell lysate, A549 cell lysate, MCF-7 cell lysate, A549, human kidney tissue, human skin tissue. Subcellular Location: Nucleus, centromere.

CENPB is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes and is considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.

Specifications

• Antigen: CENPB
• Classification: Monoclonal
• Concentration: 2 mg/mL
• Formulation: TBS with 0.2% BSA, 50% glycerol and 0.05% sodium azide; pH 7.4
• Gene Accession No.: P07199
• Gene Symbols: CENPB
• Immunogen: Recombinant protein within human CENPB aa 1-200/599.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: A5F5
• Conjugate: Unconjugated
• Gene: CENPB
• Gene Alias: CENPB; CENP-B; CENP-B protein; centromere autoantigen B; centromere protein B; centromere protein B, 80kDa; major centromere autoantigen B
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 1059
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov