Invitrogen™ CENPE Monoclonal Antibody (1H12)

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
01-675-178	Invitrogen™ MA15758	100 μL
PIMA518025	Invitrogen™ MA518025	50 μL

Description

This target has a predicted molecular weight of 312 kDa and is only suitable for Western blot if IP is performed first.

14-3-3 sigma (stratifin, SFN, epithelial cell marker protein 1) is a member of a highly conserved family of 14-3-3 proteins that are present in all eukaryotic organisms. There are 7 known mammalian 14-3-3 isoforms epsilon, beta, zeta, gamma, theta, tau and they play important roles in many biological activities by binding to and altering the subcellular localization and/or stability of key molecules in various signaling cascades. 14-3-3 sigma was originally characterized as a human mammary epithelium marker and later rediscovered as an important molecule for cell cycle checkpoint regulation. Recently it has been reported that 14-3-3 sigma may serve as a prognosis marker predicting survival of pancreatic cancer patient treatment. 14-3-3 sigmais the only 14-3-3 isoform induced by the tumor suppressor protein p53, in response to <FONT face=Symbol>g</FONT>-irradiation and other DNA-damaging agents. 14-3-3 sigmais a p53-regulated inhibitor of G2/M progression and acts as a tumor suppressor gene that is inactivated by methylation of its 5' CpG islands in epithelial tumor cells. Two isoforms of human 14-3-3 sigma are produced by alternative splicing.

Specifications

• Antigen: CENPE
• Concentration: 1 mg/mL
• Applications: Flow Cytometry, Immunoprecipitation, Western Blot, Immunocytochemistry
• Conjugate: Unconjugated
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with 0.1% sodium azide
• Gene Accession No.: Q02224
• Gene ID (Entrez): 1062
• Immunogen: Recombinant full length CENP-E protein (human) from SP2/0 cells.
• Product Type: Antibody

• Clone: 1H12
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG κ
• Target Species: Human
• Gene: CENPE
• Gene Alias: 312kDa; AU019344; BC049989; C530022J18; Cenpe; CENP-E; centromere autoantigen E; Centromere autoantigen E (312kD); centromere protein E; centromere protein E, 312kDa; centromere-associated protein E; centromeric protein E; KIF10; kinesin 10; kinesin family member 10; kinesin superfamily protein 10; Kinesin-7; Kinesin-related protein CENPE; MCPH13; Motor domain of KIF10; N-7 kinesin; PPP1R61; protein phosphatase 1, regulatory subunit 61
• Gene Symbols: CENPE
• Primary or Secondary: Primary
• Purification Method: Ion-exchange chromatography