MilliporeSigma™ Cep152 (P7), Rabbit, Unlabeled, Polyclonal,

Catalog No.	Quantity	Conjugate
ABE1977MI	100 μL	Unconjugated

Catalog No. ABE1977MI Supplier MilliporeSigma™ Supplier No. ABE1977

Description

Rabbit Polyclonal Antibody

Centrosomal protein of 152kDa (UniProt O94986; also known as Cep152) is encoded by the CEP152 (also known as KIAA0912, MCPH9, SCKL5) gene (Gene ID 22995) in human. The mammalian Asl (Asterless) ortholog Cep152 is a centrosomal protein (CEP) that plays an important role in centriole duplication and centriole-to-centrosome conversion. The centrosome is the main microtubule-organizing center essential spindle formation and chromosome segregation during mitosis. Centrosomes are composed of two orthogonally arranged centrioles surrounded by pericentriolar material (PCM). Centriole duplication begins by assembling a procentriole in G1/S phase, where centrosomal scaffolds Cep152 and Cep192 (Spd-2 ortholog) mediate the recruitment of polo kinase Plk4 to to centrosomes, a key event that triggers the entire duplication process. New centrioles assemble perpendicularly to pre-existing ones in G1/S and elongate throughout S and G2. The conversion of fully elongated daughter centrioles into centrosomes during mitotic progression requires sequential loading of Cep135, Cep295 (Ana1 analog) and Cep152 onto daughter centrioles.Cep152 is also an important regulator of genomic integrity and cellular response to DNA damage. CEP152 gene mutations are identified among individuals with Seckel syndrome, a heterogeneous autosomal recessive disorder characterized by a proportionate short stature, severe microcephaly and mental retardation, and a typical &primebird-head&prime facial appearance. Cep152 impairment in Seckel patients-derived cells leads to accumulation of genomic defects as a reuslt of replicative stress through enhanced activation of ATM signaling and increased H2AX phosphorylation.

Specifications

• Antigen: Cep152 (P7)
• Applications: Western Blot
• Classification: Polyclonal
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified rabbit polyclonal antibody in buffer containing 80mM Tris (pH 8.0), 40mM glycine, and 50% ethylene glycol with 0.05% Sodium Azide.
• Gene: CEP152, KIAA0912, MCPH9, SCKL5
• Gene Accession No.: O94986
• Host Species: Rabbit
• Immunogen: Recombinant human Cep152 C-terminal fragment.
• Purification Method: Affinity Purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Epigenetics & Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001181927
• Test Specificity: This rabbit polyclonal antibody immunostains centrisomes by indirect fluorescence and detected shRNA-mediated Cep152 knockdown by Western blotting. Target C-terminal region is present in human Cep152 spliced isoforms 3 and 4, but more than 95% absent in spliced isoforms 1 and 2 reported by UniProt (O94986).
• Target Species: Human
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

For Research Use Only. Not for use in diagnostic procedures.