Invitrogen™ CHFR Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA530655

Catalog No. PIPA530655

Description

Recommended positive controls: 293T. Predicted reactivity: Human (99%), Mouse (85%), Rat (84%), Bovine (81%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.

Specifications

• Antigen: CHFR
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Chfr
• Gene Alias: 5730484M20Rik; C230082M18; checkpoint with forkhead and ring finger domains; Checkpoint with forkhead and RING finger domains protein; checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase; Chfr; E3 ubiquitin-protein ligase CHFR; fc43f10; FLJ10796; FLJ33629; RING finger protein 196; RING-type E3 ubiquitin transferase CHFR; RNF116; RNF196; si:dkey-69h6.7; wu:fc43f10
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 55743
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q96EP1
• Gene Symbols: Chfr
• Immunogen: Recombinant protein fragment corresponding to a region within amino acids 351 and 538 of Human CHFR.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG