Invitrogen™ Human CHI3L1/YKL-40 ELISA Kit

Description

ELISA

The Human CHI3L1/YKL-40 ELISA quantitates Hu CHI3L1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CHI3L1. Principle of the method The Human CHI3L1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Human cartilage glycoprotein 39 (GP-39), also known as YKL-40, is a glycoprotein secreted by articular chondrocytes, synoviocytes and macrophages. Serum and synovial fluid GP-39 levels are elevated in inflammatory diseases and correlate with the degree of joint destruction in rheumatoid arthritis. GP-39 is expressed in articular chondrocytes and synovial cells, as well as in liver, but is undetectable in muscle tissues, lung, pancreas, mononuclear cells and fibroblasts. GP-39 is a candidate autoantigen in rheumatoid arthritis and is important in the capacity of cells to respond to and cope with changes in their environment.

Specifications

• Accession Number: P36222
• Assay Range: 6.14 to 1500 pg/mL
• Assay Sensitivity: 6 pg/mL
• Calibration Range: 6 pg/mL to 1500 pg/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Product Type: ELISA
• Protein Family: Kinase & Inhibitors
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA pair antibody detects human CHI3L1. Other species not determined.
• Sufficient For: 5 x 96 Assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 1116
• Gene Symbol: ASRT7,CGP-39,GP-39,GP39,HC-gp39,HCGP-3 P,YKL-40,YKL40,YYL-40,hCGP-39
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Angiogenesis
• Sample Volume: Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Synonym: ASRT7, CGP-39, GP-39, GP39, HC-gp39, HCGP-3 P, YKL-40, YKL40, YYL-40, hCGP-39
• Target: CHI3L1
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins