Chlamydial MOMP, Mouse anti-Bacteria, Clone: L21-10, Millipore Sigma™

Catalog No.	Quantity
MABF2166100	100 μg
MABF216625	25 μg

Catalog No. MABF2166100 Supplier MilliporeSigma Supplier No. MABF2166100UG

Description

Mouse Monoclonal Antibody

Anti-Chlamydial MOMP, clone L21-10, Cat. No. MABF2166, is a mouse monoclonal antibody that detects Chlamydial major outer membrane protein (MOMP) and has been tested for use in Dot Blot, Immunofluorescence, Immunoprecipitation, and Western Blotting.

Chlamydia trachomatis is a Gram-negative bacterium that is responsible for sexually transmitted diseases leading to pelvic inflammatory disease, ectopic pregnancy, infertility, and outbreaks of trachoma-associated blindness and lymphogranuloma venereum (LGV). Chlamydia trachomatis consists of eighteen different serological variants (serovars) that include a few subvariants. These are identified based on serological reactivity of the epitopes on their outer membrane. Intracellularly chlamydia replicates within a vacuole. Chlamydia infection is initiated with the expression of a chlamydial early gene product(s), which isolate the inclusion from the endocytic-lysosomal pathway and makes it fusogenic with sphingomyelin-containing exocytic vesicles. This change in vesicular interaction allows the delivery of the vacuole to the peri-Golgi region of the host cell. Antigens from all members of the Chlamydia genus display heat resistance and sensitivity to oxidation by sodium periodate. Clone L21-10 specifically detects major outer membrane protein (MOMP) from Chlamydia genus. It displays strong reactivity with Chlamydial serovars D and L3 and weak reactivity with Ba, E, G, L1, and L2. MOMP, a cysteine-rich molecule, plays a vital role in maintaining the structural and functional properties of the chlamydial outer membrane. It contains determinants that confer type, subspecies, and species antigenic properties to the protein. (Ref.: Zhang, YX et al., (1987). J. Immunol. 138(2); 575-581).

Specifications

• Antigen: Chlamydial MOMP
• Applications: Dot Blot, Immunofluorescence, Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: L21-10
• Conjugate: Unconjugated
• Dilution: Immunoprecipitation Analysis: A representative lot immunoprecipiated Chlamydial MOMP in Immunoprecipitation applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Immunofluorescence Analysis: A representative lot detected Chlamydial MOMP in Immunofluorescence applications (Weber, M.M., et. al. (2017). Cell Rep. 19(7):1406-1417; Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Western Blotting Analysis: A representative lot detected Chlamydial MOMP in Western Blotting applications (Baehr, W., et. al. (1988). Proc Natl Acad Sci USA. 85(11):4000-4; Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Dot Blot Analysis: A representative lot detected Chlamydial MOMP in Dot Blot applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).
• Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Alias: Chlamydia major membrane protein
• Gene Symbols: N/A
• Host Species: Mouse
• Immunogen: Formalin killed Chlamydia trachomatis (L2/LGV-434).
• Purification Method: Protein G purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Inflammation & Immunology
• Primary or Secondary: Primary
• Test Specificity: Clone L21-10 specifically detects outer membrane protein from Chlamydia genus. It displays strong reactivity with serovars D and L3 and weak reactivity with Ba, E, G, L1, and L2.
• Target Species: Bacteria
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Form: Purified
• Isotype: IgG2a λ