Invitrogen™ CHMP3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA587794

Catalog No. PIPA587794

Description

Immunogen sequence: MGLFGKTQEK PPKELVNEWS LKIRKEMRVV DRQIRDIQRE EEKVKRSVKD AAKKGQKDVC IVLAKEMIRS RKAVSKLYAS KAHMNSVLMG MKNQLAVLRV AGSLQKSTEV MKAMQSLVKI PEIQATMREL SKEMMKAGII EEMLEDTFES MDDQEEMEEE AEMEIDRILF EITAGALGKA PSKVTDALPE PEPPGAMAAS EDEEEEEEAL EAMQSRLATL RS; Positive Samples: BT-474, LO2, 293T, SW480; Cellular Location: Cytoplasm, Endosome, Late endosome membrane, Lipid-anchor, Membrane, cytosol.

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Plays a role in endosomal sorting/trafficking of EGF receptor. Isoform 2 prevents stress- mediated cell death and accumulation of reactive oxygen species when expressed in yeast cells.

Specifications

• Antigen: CHMP3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CHMP3
• Gene Alias: 25.1; 25.1 protein; 4921505F14Rik; 9130011K15Rik; CGI149; CGI-149; CGI-49; Charged multivesicular body protein 3; CHMP family, member 3; CHMP3; Chromatin-modifying protein 3; D6Ertd286e; hVps24; NEDF; Neuroendocrine differentiation factor; rVps24p; vacuolar protein sorting 24; vacuolar protein sorting 24 homolog; vacuolar protein sorting-associated protein 24; vacuolar protein-sorting-associated protein 24; Vps24; Vps24p protein
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 51652, 66700
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 2.29 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: Q9CQ10, Q9Y3E7
• Gene Symbols: CHMP3
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-222 of human VPS24 (NP_0571631).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG