Tocris Bioscience™ Chroman 1

Description

Highly potent and selective ROCK 2 inhibitor; improves cell survival after cryogeneisis

Chroman 1 is a highly potent and selective ROCK2 (Rho-kinase) inhibitor (IC50 values are 1 and 52 pM at ROCK2 and ROCK1, respectively). Chroman 1 exhibits >2000-fold selectivity for ROCK2 over a range of related kinases including MRCK, PKA, and AKT1 (IC50 values are 150, >20000, and >20000 nM, respectively). Chroman 1 promotes survival of pluripotent stem cells (PSCs) in culture when used in combination with Emricasan (Cat. No. 7310), Polyamines, and Trans-ISRIB (Cat. No. 5284), a combination known as CEPT. When used as part of the CEPT cocktail, Chroman 1 also improves survival of differentiated cells following cryopreservation with cardiomyocyte cell survival increasing by 36% and motor neuron survival improving by 63% compared with DMSO controls.

Specifications

• Chemical Name or Material: (3 S)-N-[2-[2-(Dimethylamino)ethoxy]-4-(1 H-pyrazol-4-yl)phenyl]-3,4-dihydro-6-methoxy-2 H-1-benzopyran-3-carboxamide dihydrochloride
• Quantity: 50 mg
• Target: Rho-kinase Inhibitors
• Molecular Formula: C₂₄H₂₈N₄O₄0.2 HCl
• Formula Weight: 12373
• Purity: 98%