Chromogranin A Antibody (CGA/414), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 4 publications

Catalog Number	Mfr. No.	Quantity
NBP229428	Novus Biologicals NBP229428	0.1 mg

Description

Chromogranin A Monoclonal specifically detects Chromogranin A in Human, Mouse samples. It is validated for Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Chromogranin A
• Concentration: 0.2 mg/ml
• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Apoptosis, Cancer, Neuronal Cell Markers
• Gene Accession No.: P10645
• Gene ID (Entrez): 1113
• Immunogen: Recombinant full-length human Chromogranin A protein (Uniprot: P10645)
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.

• Clone: CGA/414
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: betagranin (N-terminal fragment of chromogranin A), CGA, chromogranin A (parathyroid secretory protein 1), chromogranin-A, parathyroid secretory protein 1, Pituitary secretory protein I, SP-I
• Gene Symbols: CHGA
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.

For Research Use Only