Chromogranin A Antibody (CHGA/765) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP247847A	Novus Biologicals NBP2478470.1MG	0.1 mg
NBP247847B	Novus Biologicals NBP2478470.2MG	0.2 mg

Description

Chromogranin A Monoclonal specifically detects Chromogranin A in Human, Rat (Negative) samples. It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Chromogranin A
• Concentration: 1.0 mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Apoptosis, Cancer, Neuronal Cell Markers, Tumor Biomarkers
• Formulation: PBS with No Preservative
• Gene ID (Entrez): 1113
• Immunogen: Recombinant human chromogranin A protein
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

• Clone: CHGA/765
• Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Rat (Negative)
• Gene Alias: betagranin (N-terminal fragment of chromogranin A), CGA, chromogranin A (parathyroid secretory protein 1), chromogranin-A, parathyroid secretory protein 1, Pituitary secretory protein I, SP-I
• Gene Symbols: CHGA
• Isotype: IgG2a κ
• Purification Method: Protein A or G purified
• Test Specificity: Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.