Chromogranin A Mouse, Clone: SPM339, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP232957F	Novus Biologicals NBP2329570.02MG	0.02 mg
NBP232957A	Novus Biologicals NBP2329570.1MG	0.1 mg
NBP232957B	Novus Biologicals NBP2329570.2MG	0.2 mg

Description

Chromogranin A Monoclonal specifically detects Chromogranin A in Human, Mouse, Rat, Porcine, Monkey, Guinea Pig (Negative), Rabbit (Negative), Sheep (Negative) samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Chromogranin A
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Pig, Primate, Guinea Pig (Negative), Rabbit (Negative), Sheep (Negative)
• Gene Accession No.: P10645
• Gene ID (Entrez): 1113
• Immunogen: Human pheochromocytoma
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.

• Clone: SPM339
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Apoptosis, Cancer, Neuronal Cell Markers
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: betagranin (N-terminal fragment of chromogranin A), CGA, chromogranin A (parathyroid secretory protein 1), chromogranin-A, parathyroid secretory protein 1, Pituitary secretory protein I, SP-I
• Gene Symbols: CHGA
• Isotype: IgG1 κ
• Purification Method: Protein A purified
• Test Specificity: Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.