Invitrogen™ CLCN3 Monoclonal Antibody (N258/5), PerCP

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA545556

Catalog No. PIMA545556

Description

1 μg/mL of MA5-45556 was sufficient for detection of Clcn3 in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 90kDa. Does not cross-react with Clcn4 or Clcn5 (based on KO validation results). This antibody was formerly sold as clone S258-5.

Mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory.

Specifications

• Antigen: CLCN3
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 2.48mM MES, 95.64mM phosphate with 0.5M EDTA and no preservative; pH 7.4
• Gene Accession No.: P51790, P51791, P51792
• Gene Symbols: CLCN3
• Immunogen: Synthetic peptide amino acids 98-115 (cytoplasmic N-terminus) of rat Clcn3.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunohistochemistry, Western Blot, Immunocytochemistry
• Clone: N258/5
• Conjugate: PerCP
• Gene: CLCN3
• Gene Alias: chloride channel 3; chloride channel protein 3; chloride channel, voltage-sensitive 3; chloride transporter ClC-3; chloride voltage-gated channel 3; CIC3; Clc3; clC-3; CLCN 3; Clcn3; DKFZp564I0463; H(+)/Cl(-) exchange transporter 3; protein kinase C-regulated chloride channel
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 1182, 12725, 84360
• Content And Storage: 4°C
• Form: Liquid

Safety and Handling

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