Invitrogen™ CLEC2D Polyclonal Antibody

Goat Polyclonal Antibody

Supplier:  Invitrogen™ PA547496

Catalog No. PIPA547496

Description

In Western blots, approximately 15% cross-reactivity with recombinant mouse (rm) OCIL is observed and less than 1% cross-reactivity with rmOCILrp2, recombinant human (rh) CLEC4D, and rhCLEC4F is observed. Reconstitute at 0.2 mg/mL in sterile PBS.

Recently, Zhou et al have identified a protein that can inhibit multinucleate Osteoclast formation in murine osteoblast and spleen cell co-cultures. This protein called, Osteoclast Inhibitory Lectin (OCIL) can also inhibit osteoclast formation in spleen cell cultures treated with RANKL and M-CSF. Furthermore, mOCIL acted directly on macrophage/monocyte cells as evidenced by its inhibitory action on adherent spleen cell cultures, which were depleted of stromal and lymphocytic cells. mOCIL completely inhibited osteoclast formation during the proliferative phase of osteoclast formation and resulted in 70% inhibition during the differentiation phase. Osteoblast OCIL mRNA expression was enhanced by parathyroid hormone, calcitriol, interleukin-1 and IL-11, and retinoic acid. OCIL is expressed in osteoblasts and chondrocytes as well as in a variety of extraskeletal tissues. Since OCIL expression was found to be very similar to distribution for RANKL, it has been suggested that RANKl and OCIL may interact in skeleton and in extracellular tissues.

Specifications

• Antigen: CLEC2D
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CLEC2D
• Gene Alias: CLAX; Clec2d; Clrb; Clr-b; C-type lectin domain family 2 member D; C-type lectin domain family 2, member d; C-type lectin related f; C-type lectin superfamily 2, member D; C-type lectin-domain family 2 member D; C-type lectin-related protein B; lectin-like NK cell receptor; Lectin-like transcript 1; lectin-like transmembrane protein; LLT1; LLT-1; MGC123478; OCIL; Osteoclast inhibitory lectin
• Host Species: Goat
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 29121
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized

• Applications: Flow Cytometry, Western Blot
• Concentration: 0.2 mg/mL
• Formulation: PBS with 5% trehalose and No Preservative
• Gene Accession No.: Q9UHP7
• Gene Symbols: CLEC2D
• Immunogen: E. coli-derived recombinant human OCIL/CLEC2d Ser57-Val191.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG