Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Molecular Probes™ Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit Non-distribution product as customer accommodation. Greener Choice Available on GSA/VA Contract for Federal Government customers only.

Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit

Manufacturer:  Molecular Probes™ C10456

Catalog No. C10456

Add to cart



The Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit provides a fast, sensitive, and non-radioactive method for the detection of protein synthesis using fluorescence microscopy or high-content imaging. In this assay O-propargyl-puromycin (OPP) is efficiently incorporated into newly translated proteins in complete methionine-containing media and fluorescently labeled with a bright, photostable Alexa Fluor™ dye in a fast, highly-specific, and mild click reaction.

Features of the kit include:

  • No media change required—works in complete, methionine-containing media, no need to remove cell media
  • Multiplex-enabled—Click-iT™ Plus technology retains signal from GFP and binding of fluorescent-conjugated phalloidins
  • Non-radioactive—an alternative to the traditional 35S-methionine methods
  • Works in vivo—published results demonstrate use in vivo for determination of protein translation

    The kit contains O-propargyl-puromycin (OPP), which is an alkyne analog of puromycin (also available separately), as well as Alexa Fluor™ 488 picolyl azide and all necessary reagents to perform the Click reaction. The OPP is fed to cultured cells and incorporated into proteins during active protein synthesis. Addition of the Alexa Fluor™ 488 picolyl azide and the Click reaction reagents leads to a chemoselective ligation, or 'click' reaction, between the picolyl azide dye and the OPP alkyne, allowing the modified proteins to be detected by imaged-based analysis.

    The click reaction uses bioorthogonal (biologically unique) moieties to fluorescently label proliferating cells, helping produce low backgrounds and high detection sensitivities. Because of the mild reaction conditions, Click-iT™ Plus assays detect protein translation events while enabling the preservation of cell morphology, the binding of fluorescently-labeled phalloidin, and the fluorescent signal from GFP.

    Unlike 35S-methionine, used in traditional methods, OPP is not an amino acid analog, so it can be added directly to cells in complete media or used to determine protein synthesis in vivo.

    The kit contains all of the components needed to label and detect the incorporated OPP in newly translated proteins in samples of adherent cells. The kit includes sufficient reagents for the labeling of 25 18 mm x 18 mm coverslips using 1 mL of reaction buffer per test.

    This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

  • Specifications


    This kit contains: Click-iT® OPP Reagent, Alexa Fluor® dye picolyl azide, Click-iT® OPP Reaction Buffer, Copper Protectant, Click-iT® Reaction Buffer Additive, Click-iT® Reaction Rise Buffer, and NuclearMask™ Blue Stain.

    Store at 2–8°C. Desiccate and protect from light.
    Nascent Protein Labeling Reagent
    Provide Content Correction

    The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

    Product Title

    By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

    Cancel Submit

    For Research Use Only. Not for use in diagnostic procedures.