CLM-8 (CD300a) Mouse anti-Human, Alexa Fluor 647, Clone: MEM-260, BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 566342
The MEM-260 monoclonal antibody specifically recognizes CD300a which is also known as CMRF35-like molecule 8 (CLM8), Inhibitory receptor protein 60 (IRp60), or Immunoglobulin superfamily member 12 (IGSF12). CD300a is a ñ60 kDa type I transmembrane glycoprotein that belongs to the CMRF family within the Ig gene superfamily. CD300a is expressed on natural killer (NK) cells, T and B cell subsets, monocytes, macrophages, dendritic cells, granulocytes and mast cells. CD300a has a single Ig V-like extracellular domain which is homologous to the Ig-binding domains of the of the Fc receptor for polymeric IgA and IgM. CD300a is a non-MHC-specific inhibitory receptor that contains three immunoreceptor tyrosine-based inhibitory motifs in its intracellular region. CD300a can reportedly inhibit NK cell cytolytic activity and granulocyte and mast cell functions.Alexa Fluor™ 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor™ 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor™ 647 cannot be used simultaneously.
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|CD300A, CLM-8, CLM8, CMRF-35-H9, CMRF35H, IGSF12, IRp60, IRC1: IRC2|
|HPB Human Acute Lymphoid Leukemia Cell Line|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor™ 647 under optimum conditions, and unreacted Alexa Fluor™ 647 was removed.|
|Alexa Fluor 647|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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