Invitrogen™ CNTF Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P140100UG

Catalog No. 500P140100

Description

AA Sequence of recombinant protein: AFTEHSPLTP HRRDLCSRSI WLARKIRSDL TALTQSYVKH QGLNKNINLD SADGMPVAST DQWSQLTQAQ RLQQNLQAYR TFHVLLARLL QDQQVHFTPT QGDFHQAIHT LLLQVAAFAY QIQQLMILLQ YKIPRNQADG MPINVGDGGL FQKKLWGLKV LQQLSQWTVR SIHDLRFISS HQTGIPARGS HYIANNKKM. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human CNTF. Anti-Human CNTF-specific antibody was purified by affinity chromatography employing an immobilized Human CNTF matrix. Sandwich ELISA: To detect Human CNTF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human CNTF (500-P140Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human CNTF. Western Blot: To detect Human CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of ZFP91 and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse.

Specifications

• Antigen: CNTF
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CNTF
• Gene Alias: AI429687; Ciliary neurotrophic factor; ciliary neurotropic factor; Cntf; HCNTF; H-CNTF
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1270
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P26441
• Gene Symbols: CNTF
• Immunogen: E.coli-derived Recombinant Human CNTF.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody