Novus Biologicals™ Coagulation Factor X Antibody Pair [HRP]

Description

Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Coagulation Factor X.

Specifications

• Assay Range: 5.47 pg/mL
• Assay Sensitivity: 5.47-350 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Coagulation Factor X.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: coagulation factor X, EC 3.4.21, EC 3.4.21.6, factor Xa, FX, FXA, prothrombinase, Stuart factor, Stuart-Prower factor
• Gene ID (Entrez): 2159
• Gene Symbol: F10
• Host Species: Rabbit/Mouse
• Kit Contents:
  Standard: Each vial contains 30 ng of recombinant Human Coagulation Factor X.
  Capture Antibody: 1 mg/mL of mouse anti-Human Coagulation Factor X monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.2 mg/mL of rabbit anti-Human Coagulation Factor X monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Storage Requirements: Storage is content dependent.
• Target: Coagulation Factor X
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 350 pg/mL is recommended.