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  6. S05963

EDVOTEK™ Code Breakers: Using CRISPR to Rewrite Genetics
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Catalog No. S05963
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Class Size:
10 Lab Groups

Description

In this 'Code Breakers' experiment, students explore cutting-edge genetic engineering using CRISPR-Cas9 to knock out the purple chromogenic genes in classroom safe bacteria. Additionally, students test the specificity of CRISPR-Cas9 by using a non-specific CRISPR gRNA template.

  • Explore genetic engineering, CRISPR, and the central dogma of molecular biology in this comprehensive experiment.
  • Transform classroom- safeE. coli with CRISPR RNA templates to test the targeting specificity of the technology.
  • Identify successful gene knockout based on the color of the cells.
  • Group Size: For 10 Lab Groups
  • Time Required: Set Up - 50 minutes / Incubation - overnight / Transformation - 90 minutes
  • Kit Includes: Instructions, LyphoCells™E.coli Purple, ampicillin, IPTG, Chloramphenicol, ReadyPour™ SOC-agar, pCas9-PurpleGuide (plasmid), pCas9-ControlGuide (plasmid), CaCl2, competent cell solution, SOC liquid medium (broth), SOC additive (for ReadyPour ONLY), petri plates (small), petri plates (large), plastic microtipped transfer pipets, wrapped 10 ml pipet (sterile), inoculating loops (sterile), microcentrifuge tubes, 50mL conical tubes
  • All You Need:Automatic micropipette (5-50 μl) and tips, two water baths (37°C and 42°C), incubation oven (optional but recommended), thermometer, centrifuge(s), vortexer (optional), floating racks, pipet pumps or bulbs, ice, marking pens, tape, hot plate or microwave oven, and hot gloves
  • Storage: Some Components Require Freezer Storage Upon Receipt.

Specifications

Product Type Biotechnology Kit
Grade 9 to Undergraduate
Class Size 10 Lab Groups
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