MilliporeSigma™ Cofilin-1, Rabbit, Unlabeled, Polyclonal,

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ ABT1493

Catalog No. ABT1493

Description

Cofilin-1 (UniProt P23528; also known as 18kDa phosphoprotein, Cofilin non-muscle isoform, p18) is encoded by the CFL1 (also known as CFL) gene (Gene ID 1072) in human. Cofilin-1 (non-muscle cofilin), cofilin-2 (muscle cofilin), and actin-depolymerizing factor (ADF) play an essential role in regulating actin filament dynamics and reorganization by stimulating the severance and depolymerization of actin filaments. The three cofilin family members share 70% to 81% sequence identity, exhibit similar biological functions, and display distinct but overlapping expression patterns. Cofilins and ADF bind actin monomers and filaments (G-actin and F-actin) and increase the number of actin monomers and filament fragments, facilitating filament turnover and treadmilling at key locations in migrating cells. Cofilins and ADF are inactivated by phosphorylation at Ser3 by LIM-kinases (LIMKs) and testicular protein kinases (TESKs) and are reactivated by dephosphorylation by the slingshot (SSH) family of protein phosphatases and chronophin. Cfl1-knockout results in mouse embryonic lethality at E11.5 12.5 due to aberrant neural tube closure and defective neural crest cell migration, while Adf-deficient mice are viable with normal brain appearance, but suffer from corneal defects that cause blindness in adult mice.

Specifications

• Antigen: Cofilin-1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CFL, CFL1
• Host Species: Rabbit
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This rabbit polyclonal antibody targets cofilin-1 N-terminal end sequence conserved among bovine, human, mouse, porcine, and rat species.
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.

• Applications: Western Blot
• Concentration: Please refer to lot specific datasheet.
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene Accession No.: P23528
• Immunogen: KLH-conjugated linear peptide corresponding to the N-termnal end sequence of human cofilin-1.
• Quantity: 100 μL
• Research Discipline: Cell Structure
• Gene ID (Entrez): NP_005498
• Target Species: Human

For Research Use Only. Not for use in diagnostic procedures.