Invitrogen™ COL4A3 Polyclonal Antibody

Goat Polyclonal Antibody

Supplier:  Invitrogen™ PA537854

Catalog No. PIPA537854

Description

This antibody is predicted to react with dog and cow based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.

Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jun 2010].

Specifications

• Antigen: COL4A3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: COL4A3
• Gene Alias: COL4A3; collagen alpha-3; Collagen alpha-3(IV) chain; collagen IV, alpha-3 polypeptide; collagen type IV alpha 3 chain; collagen, type IV, alpha 3 (Goodpasture antigen); Goodpasture antigen; IV chain; Tumstatin
• Host Species: Goat
• Purification Method: Ammonium sulfate precipitation
• Regulatory Status: RUO
• Gene ID (Entrez): 1285
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin)
• Concentration: 0.5 mg/mL
• Formulation: TBS with 0.5% BSA and 0.02% sodium azide; pH 7.3
• Gene Accession No.: Q01955
• Gene Symbols: COL4A3
• Immunogen: Peptide with sequence C-RGPTEYYDTYQEK, from the internal region of COL4A3 (aa341-353).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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