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Collagen I alpha 1 Antibody - Azide and BSA Free, Novus Biologicals™
SDP

Catalog No. NBP13005402 Shop All R&D Systems Products
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Description

Rabbit Polyclonal Antibody has been used in 36 publications

Collagen I alpha 1 Polyclonal specifically detects Collagen I alpha 1 in Human, Mouse, Rat, Amphibian, Avian, Mammal, Sheep samples. It is validated for Western Blot, Simple Western, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

Specifications

Antigen Collagen I alpha 1
Applications Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Classification Polyclonal
Concentration 1.0 mg/mL
Conjugate Unconjugated
Dilution Western Blot 1:1000, Simple Western 1:100, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:100, Immunohistochemistry-Frozen 1:10-1:500
Formulation PBS with No Preservative
Gene Accession No. P02452
Gene Alias Alpha-1 type I collagen, Collagen 1, Collagen 1 alpha 1, collagen alpha 1 chain type I, collagen alpha-1(I) chain, collagen of skin, tendon and bone, alpha-1 chain, collagen, type I, alpha 1, Collagen1, Collagen-1, OI4, pro-alpha-1 collagen type 1
Gene Symbols COL1A1
Host Species Rabbit
Immunogen Synthetic peptide corresponding to amino acid residues specific to the collagen 1, alpha 1 telopeptide conjugated to KLH
Molecular Weight of Antigen 140 kDa
Purification Method Affinity Purified
Quantity 0.02 mL
Regulatory Status RUO
Research Discipline Cytoskeleton Markers, Extracellular Matrix
Primary or Secondary Primary
Gene ID (Entrez) 1277
Test Specificity Specific for the ∼ 140 kDa telopeptide portion of the collagen I alpha 1 polypeptide. The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 degrees C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehydefixed fibrotic mouse lung tissue, the antibody recognizes mature collagen I that has formed fibrils in the extracellular matrix.
Target Species Human, Mouse, Rat, Sheep
Content And Storage Store at -20C. Avoid freeze-thaw cycles.
Isotype IgG
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