Collagen I alpha 1 Antibody - Azide and BSA Free, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 36 publications

Supplier:  Novus Biologicals NBP1300540.02ML

Catalog No. NBP13005402

Description

Collagen I alpha 1 Polyclonal specifically detects Collagen I alpha 1 in Human, Mouse, Rat, Amphibian, Avian, Mammal, Sheep samples. It is validated for Western Blot, Simple Western, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

Specifications

• Antigen: Collagen I alpha 1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: PBS with No Preservative
• Gene Alias: Alpha-1 type I collagen, Collagen 1, Collagen 1 alpha 1, collagen alpha 1 chain type I, collagen alpha-1(I) chain, collagen of skin, tendon and bone, alpha-1 chain, collagen, type I, alpha 1, Collagen1, Collagen-1, OI4, pro-alpha-1 collagen type 1
• Host Species: Rabbit
• Molecular Weight of Antigen: 140 kDa
• Quantity: 0.02 mL
• Research Discipline: Cytoskeleton Markers, Extracellular Matrix
• Gene ID (Entrez): 1277
• Target Species: Human, Mouse, Rat, Sheep
• Isotype: IgG

• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 1:1000, Simple Western 1:100, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:100, Immunohistochemistry-Frozen 1:10-1:500
• Gene Accession No.: P02452
• Gene Symbols: COL1A1
• Immunogen: Synthetic peptide corresponding to amino acid residues specific to the collagen 1, alpha 1 telopeptide conjugated to KLH
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Specific for the ∼ 140 kDa telopeptide portion of the collagen I alpha 1 polypeptide. The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 degrees C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehydefixed fibrotic mouse lung tissue, the antibody recognizes mature collagen I that has formed fibrils in the extracellular matrix.
• Content And Storage: Store at -20C. Avoid freeze-thaw cycles.