Collagen I Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 195 publications

Supplier:  Novus Biologicals NB6004080.025MG

Catalog No. NB600408025

Description

Collagen I Polyclonal specifically detects Collagen I in Human, Mouse, Rat, Porcine, Bacteria, Bovine, Rabbit samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, SDS-Page, Fluorophore-linked immunosorbent assay, Microarray.

Specifications

• Antigen: Collagen I alpha 1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
• Gene Alias: Alpha-1 type I collagen, Collagen 1, Collagen 1 alpha 1, collagen alpha 1 chain type I, collagen alpha-1(I) chain, collagen of skin, tendon and bone, alpha-1 chain, collagen, type I, alpha 1, Collagen1, Collagen-1, OI4, pro-alpha-1 collagen type 1
• Host Species: Rabbit
• Molecular Weight of Antigen: 139 kDa
• Quantity: 0.025 mg
• Research Discipline: Cancer, Cell Cycle and Replication, Cytoskeleton Markers, Extracellular Matrix
• Gene ID (Entrez): 1277
• Target Species: Human, Mouse, Rat, Pig, Bovine, Feline, Rabbit
• Isotype: IgG

• Applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 1:1000, ELISA 1:5000-1:50000, Immunohistochemistry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:100, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen 1:10-1:500, Immunocytochemistry 1:1600
• Gene Accession No.: P02452, P02453
• Gene Symbols: COL1A1
• Immunogen: Collagen Type I from human and bovine placenta by limited pepsin digestion and selective salt precipitation.
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Typically less than 1% cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

For Research Use Only