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Collagen I Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 195 publications
Supplier: Novus Biologicals NB6004080.025MG
Description
Collagen I Polyclonal specifically detects Collagen I in Human, Mouse, Rat, Porcine, Bacteria, Bovine, Rabbit samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, SDS-Page, Fluorophore-linked immunosorbent assay, Microarray.Specifications
Collagen I alpha 1 | |
Polyclonal | |
Unconjugated | |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide | |
Alpha-1 type I collagen, Collagen 1, Collagen 1 alpha 1, collagen alpha 1 chain type I, collagen alpha-1(I) chain, collagen of skin, tendon and bone, alpha-1 chain, collagen, type I, alpha 1, Collagen1, Collagen-1, OI4, pro-alpha-1 collagen type 1 | |
Rabbit | |
139 kDa | |
0.025 mg | |
Cancer, Cell Cycle and Replication, Cytoskeleton Markers, Extracellular Matrix | |
1277 | |
Human, Mouse, Rat, Pig, Bovine, Feline, Rabbit | |
IgG |
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin) | |
1.0 mg/mL | |
Western Blot 1:1000, ELISA 1:5000-1:50000, Immunohistochemistry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:100, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen 1:10-1:500, Immunocytochemistry 1:1600 | |
P02452, P02453 | |
COL1A1 | |
Collagen Type I from human and bovine placenta by limited pepsin digestion and selective salt precipitation. | |
Affinity Purified | |
RUO | |
Primary | |
Typically less than 1% cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. | |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
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