Invitrogen™ Collagen I Monoclonal Antibody (5D8-G9)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA1141

Catalog No. PIMA1141

Description

MA1-141 detects collagen I from bovine, human, and porcine. This antibody will not cross react with collagen 2-11, or detect thermally denatured collagen. Western blot analysis of recombinant bovine collagen I using MA1-141 detected a predominant band around 270kD likely corresponding to the alpha-1 dimer and a fainter band at a higher MW likely corresponding to the alpha-1/alpha-2 trimer. MA1-141 has been successfully used in ELISA, immunofluorescence, immunohistochemistry (frozen and paraffin), and Western blot procedures. The epitope is sensitive to routine formalin fixation and paraffin embedding. Strong staining of connective tissue fibres is seen in acetone-fixed or unfixed frozen sections.

Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.

Specifications

• Antigen: Collagen I
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide
• Gene Accession No.: P02452, P02453, P02465, P08123
• Gene Symbols: COL1A1, COL1A2
• Immunogen: Purified, full-length native human collagen I protein.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Bovine, Pig
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
• Clone: 5D8-G9
• Conjugate: Unconjugated
• Gene: COL1A2
• Gene Alias: AA960264; AI325291; alpha 1 type I collagen; alpha 2 type I collagen; alpha 2 type I procollagen; alpha 2(I) collagen; alpha 2(I) procollagen; alpha 2(I)-collagen; alpha1 chain of type I collagen; alpha-1 collagen (I); alpha-1 type 1 collagen; alpha-1 type I collagen; alpha-1 type-1 collagen; alpha1(I) procollagen; alpha2 chain of type I collagen; alpha-2 type I collagen; alpha-2(1) collagen; alpha2(I) collagen; COL1; Col1a1; Col1a-1; COL1A1; matrix protein; Col1a2; Col1a-2; COLA; Cola1; Cola-1; Cola2; Cola-2; COLIA1; collagen; collagen 1 alpha 2 chain; collagen a2; collagen alpha 1 chain type I; collagen alpha 2(I) chain precursor; collagen alpha-1 chain; collagen alpha-1 chain precursor; collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; collagen alpha-2; collagen alpha-2 chain precursor; collagen alpha-2(I) chain; collagen COL1A2; collagen I alpha-2 polypeptide; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tendon and bone, alpha-2 chain; collagen type I alpha 1; collagen type I alpha 1 chain; collagen type I alpha 2 chain; collagen, type 1, alpha 1; collagen, type I, alpha 1; collagen, type I, alpha 2; EDSC; Mov13; Mov-13; OI1; OI2; OI3; OI4; oim; osteogenesis imperfecta; precollagen alpha-2; prepro-alpha-1 collagen type I; pro-alpha-1 collagen type 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; procollagen, type I, alpha 1; procollagen, type I, alpha 2; similar to pro alpha 1(I) collagen; Type I Collagen; type I collagen alpha 1 chain; type I collagen alpha1; type I collagen alpha-2 chain; type I collagen pre-pro-alpha1(I) chain; type I proalpha 1; type I procollagen; type I procollagen alpha 1 chain; type I-alpha 1 collagen
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 100626716, 100738123, 1277, 1278, 282187, 282188
• Content And Storage: -20°C
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov