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Invitrogen™ Collagen I Polyclonal Antibody
Rabbit Polyclonal Antibody
Supplier: Invitrogen™ PA126204
Description
Some class-specific anti-collagens are specific for three-dimensional epitopes, resulting in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Cross-reactivity with other human serum proteins and non-collagen extracellular matrix proteins is neglible. Western blot: this preparation results in a native conformation of the protein. It is recommend not to add 2-ME or DTT in lysis buffer and not boiling the sample.
Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.
Specifications
Collagen I | |
Polyclonal | |
Unconjugated | |
COL1A2 | |
AA960264; AI325291; alpha 1 type I collagen; alpha 2 type I collagen; alpha 2 type I procollagen; alpha 2(I) collagen; alpha 2(I) procollagen; alpha 2(I)-collagen; alpha1 chain of type I collagen; alpha-1 collagen (I); alpha-1 type 1 collagen; alpha-1 type I collagen; alpha-1 type-1 collagen; alpha1(I) procollagen; alpha2 chain of type I collagen; alpha-2 type I collagen; alpha-2(1) collagen; alpha2(I) collagen; COL1; Col1a1; Col1a-1; COL1A1; matrix protein; Col1a2; Col1a-2; COLA; Cola1; Cola-1; Cola2; Cola-2; COLIA1; collagen; collagen 1 alpha 2 chain; collagen a2; collagen alpha 1 chain type I; collagen alpha 2(I) chain precursor; collagen alpha-1 chain; collagen alpha-1 chain precursor; collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; collagen alpha-2; collagen alpha-2 chain precursor; collagen alpha-2(I) chain; collagen COL1A2; collagen I alpha-2 polypeptide; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tendon and bone, alpha-2 chain; collagen type I alpha 1; collagen type I alpha 1 chain; collagen type I alpha 2 chain; collagen, type 1, alpha 1; collagen, type I, alpha 1; collagen, type I, alpha 2; EDSC; Mov13; Mov-13; OI1; OI2; OI3; OI4; oim; osteogenesis imperfecta; precollagen alpha-2; prepro-alpha-1 collagen type I; pro-alpha-1 collagen type 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; procollagen, type I, alpha 1; procollagen, type I, alpha 2; similar to pro alpha 1(I) collagen; Type I Collagen; type I collagen alpha 1 chain; type I collagen alpha1; type I collagen alpha-2 chain; type I collagen pre-pro-alpha1(I) chain; type I proalpha 1; type I procollagen; type I procollagen alpha 1 chain; type I-alpha 1 collagen | |
Rabbit | |
Antigen Affinity Chromatography | |
RUO | |
1277, 1278, 12842, 12843, 282187, 282188, 29393, 84352 | |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. | |
Liquid |
ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot | |
1.15 mg/mL | |
20 mM potassium phosphate with 150 mM NaCl and 0.01% sodium azide; pH 8.0 | |
P02452, P02453, P02454, P02465, P02466, P08123, P11087, Q01149 | |
COL1A1, COL1A2 | |
Collagen type I extracted and purified from human and bovine placenta by limited pepsin digestion and selective salt precipitation. | |
100 μg | |
Primary | |
Human, Mouse, Rat, Bovine | |
Antibody | |
IgG |
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