Collagen II Antibody (5B2.5) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP280676

Catalog No. NB013749

Description

Collagen II Monoclonal specifically detects Collagen II in Human, Mouse, Rat, Chicken, Rabbit samples. It is validated for Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

Specifications

• Antigen: Collagen II
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Dilution: Western Blot : 1-5ug/ml, Immunohistochemistry reported in scientific literature (PMID 22558278; 24242000), Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen reported in scientific literature (PMID 22558278)
• Gene Alias: Alpha-1 type II collagen, ANFH, AOM, cartilage collagen, chondrocalcin, COL11A3, Collagen 2, collagen alpha-1(II) chain, collagen II, alpha-1 polypeptide, collagen type 2, collagen, type II, alpha 1, collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital), Collagen-2, MGC131516, SEDC
• Host Species: Mouse
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Rat reactivity reported in scientific literature (PMID: 24349545). Rabbit reactivity reported in scientific literature (PMID: 15621244). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
• Content And Storage: Store at 4°C. Do not freeze.
• Isotype: IgG2a κ

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Clone: 5B2.5
• Conjugate: Unconjugated
• Formulation: PBS
• Gene Symbols: COL2A1
• Immunogen: A purified preparation of lathyritic type II collagen from embryonic chicken sternum
• Quantity: 0.1 mL
• Research Discipline: Cell Biology, Cellular Markers, Extracellular Matrix, Stem Cell Markers
• Gene ID (Entrez): 1280
• Target Species: Human, Mouse, Rat, Chicken, Rabbit
• Form: Purified