Collagen III alpha 1/COL3A1 Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 21 publication

Catalog Number	Mfr. No.	Quantity
NB600594SS	Novus Biologicals NB600594SS	0.02 mg
NB600594	Novus Biologicals NB600594	0.1 mg

Description

Collagen III alpha 1/COL3A1 Polyclonal specifically detects Collagen III alpha 1/COL3A1 in Human, Mouse, Rat, Bovine, Feline samples. It is validated for Western Blot, Simple Western, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Collagen III alpha 1/COL3A1
• Dilution: Western Blot 1:1000-1:10000, Simple Western, Flow Cytometry, ELISA 1:5000-1:50000, Immunohistochemistry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoprecipitation 1:100, Immunohistochemistry-Paraffin 1:50 - 1:200
• Classification: Polyclonal
• Host Species: Rabbit
• Research Discipline: Cancer, Cell Biology, Extracellular Matrix
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
• Gene Alias: alpha1 (III) collagen, Collagen 3, collagen alpha-1(III) chain, collagen, fetal, collagen, type III, alpha 1, Collagen-3, EDS4A, Ehlers-Danlos syndrome type IV, autosomal dominant, FLJ34534
• Gene Symbols: COL3A1
• Isotype: IgG
• Purification Method: Affinity Purified
• Test Specificity: Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Collagen III alpha 1/COL3A1 and has expected cross-reactivity with Type I and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.

• Concentration: 1 mg/ml
• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Bovine, Feline
• Gene Accession No.: P02461
• Gene ID (Entrez): 1281
• Immunogen: Collagen III alpha 1/COL3A1 from human and bovine placenta (Uniprot: P02461)
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
• Molecular Weight of Antigen: 139 kDa

For Research Use Only