Collagen IV Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
NB1206586SS	Novus Biologicals NB1206586SS	0.02 mg
NB1206586	Novus Biologicals NB1206586	0.1 mg

Description

Collagen IV Polyclonal specifically detects Collagen IV in Human, Mouse, Rat, Bovine, Feline samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Knockout Validated, Super-Resolution Microscopy.

Specifications

• Antigen: Collagen IV alpha 1
• Dilution: Western Blot 1:1000-1:10000, ELISA 1:5000-1:50000, Immunohistochemistry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:100, Immunohistochemistry-Paraffin 1:50-1:200, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Knockout Validated, Super-Resolution Microscopy
• Classification: Polyclonal
• Host Species: Rabbit
• Research Discipline: Cytoskeleton Markers, Extracellular Matrix, Stem Cell Markers
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
• Gene Alias: alpha-1 chain, arresten, COL4A1 NC1 domain, collagen alpha-1(IV) chain, collagen IV, alpha-1 polypeptide, collagen, type IV, alpha 1, Collagen-4, EC 3.4.23, EC 6.3.1.2
• Gene Symbols: COL4A1
• Isotype: IgG
• Purification Method: Affinity Purified
• Test Specificity: Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Collagen IV and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

• Concentration: 1 mg/ml
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunohistochemistry (Free Floating), Immunoassay, CyTOF
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Bovine, Feline, Mammalia
• Gene Accession No.: P02462
• Gene ID (Entrez): 1282
• Immunogen: Collagen IV from human and bovine placenta (Uniprot: P02462)
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
• Molecular Weight of Antigen: 160.61 kDa

For Research Use Only