Collagen Type I Polyclonal Antibody, Rockland™

Rabbit Polyclonal Antibody

Supplier:  Rockland Immunochemicals 6004011030.5

Catalog No. 50-199-1661

Description

Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.

Specifications

• Antigen: Collagen Type I
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: COL1A1
• Gene Alias: AA960264; AI325291; alpha 1 type I collagen; alpha 2 type I procollagen; alpha 2(I) procollagen; alpha 2(I)-collagen; alpha-1 type 1 collagen; Alpha-1 type I collagen; alpha1(I) procollagen; Alpha-2 type I collagen; BOS_18945; BOS_4009; COL1; Col1a1; Col1a-1; COL1A2; Col1a-2; Cola1; Cola-1; Cola2; Cola-2; COLIA1; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; Collagen alpha-2(I) chain; collagen COL1A2; collagen I alpha-2 polypeptide; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tendon and bone, alpha-2 chain; collagen type I alpha 1; collagen type I alpha 2 chain; collagen, type 1, alpha 1; collagen, type I, alpha 1; collagen, type I, alpha 2; EDSC; LOC100347598; Mov13; Mov-13; OI1; OI2; OI3; OI4; oim; osteogenesis imperfecta; pro-alpha-1 collagen type 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; procollagen, type I, alpha 1; procollagen, type I, alpha 2; Type I
• Host Species: Rabbit
• Regulatory Status: RUO
• Gene ID (Entrez): 1277, 1278, 282187, 282188
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Accession No.: P02452, P02453, P02465, P08123
• Gene Symbols: COL1A1, COL1A2
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Bovine, Human
• Product Type: Antibody