Collagen Type IV Rabbit anti-Bovine, Human, HRP, Polyclonal, Rockland™

Catalog No.	Quantity
50-199-2398	50 μg

Catalog No. 50-199-2398 Supplier Rockland Immunochemicals Supplier No. 600403106

Description

Rabbit Polyclonal Antibody

Store vial at 4°C prior to restoration. Restore with 0.05 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration. Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Collagen IV is a major constituent of the basement membranes along with laminins and enactins. Collagen IV is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio. Collagen IV can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.

Specifications

• Antigen: Collagen Type IV
• Applications: ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: HRP
• Formulation: 0.01M sodium phosphate with 0.25M NaCl, 10 mg/mL BSA and 0.01% thimerosal; pH 7.2
• Gene: COL4A1
• Gene Accession No.: P02462, P08572, P53420, Q01955, Q28084, Q29442, Q7SIB2, Q7SIB3
• Gene Alias: [a]3(IV); [a]4(IV); alpha 1 type IV collagen; alpha1(IV) collagen; alpha-2 collagen type IV; alpha3(IV); Arresten; BOS_12713; BOS_2161; BOS_2253; Bru; BSVD; CA44; Canstatin; COL4A1; Col4a-1; COL4A1 NC1 domain; COL4A2; Col4a-2; COL4A3; COL4A4; Collagen 4; Collagen alpha-1(IV) chain; Collagen alpha-2(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; collagen IV, alpha-1 polypeptide; collagen IV, alpha-3 polypeptide; Collagen IV, alpha-4 polypeptide; collagen of basement membrane, alpha-1 chain; collagen of basement membrane, alpha-4 chain; collagen type IV alpha 1 chain; collagen type IV alpha 2; collagen type IV alpha 3 chain; collagen type IV alpha 4 chain; collagen type IV alpha3 chain; collagen, type IV, alpha 1; collagen, type IV, alpha 2; collagen, type IV, alpha 3; collagen, type IV, alpha 3 (Goodpasture antigen); collagen, type IV, alpha 4; Del(8)44H; Del(8)Bru44H; E130010M05Rik; Goodpasture antigen; ICH; POREN2; procollagen, type IV, alpha 1; procollagen, type IV, alpha 2; procollagen, type IV, alpha 3; procollagen, type IV, alpha 4; RATOR; Raw; retinal anterior wiring; Svc; Tumstatin; Type IV Collagen
• Gene Symbols: COL4A1, COL4A2, Col4a3, COL4A4
• Host Species: Rabbit
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 1282, 1284, 1285, 1286, 282191, 317711, 407107, 508632
• Target Species: Bovine, Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Lyophilized