Invitrogen™ Collagen V (soluble) Monoclonal Antibody (1E2-E4/Col5)

Catalog No.	Quantity
CSI0060102	400 μL

Catalog No. CSI0060102 Supplier Invitrogen™ Supplier No. CSI0060102

Description

Mouse Monoclonal Antibody

CSI 006-01-02 is highly specific for type V collagen. It has been shown to have no cross-reactivity with type I, III and VI collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin). CSI 006-01-02 shows reactivity with human, dog, calf, sheep, pig and kangaroo in type V collagens by ELISA and immunoblotting. CSI 006-01-02 does not show reactivity with mouse, rat, guinea pig and chicken type V collagens by ELISA and immunoblotting. CSI 006-01-02 has been successfully used in Western blotting, ELISA and Immunohistochemistry procedures. CSI 006-01 has been used succesfully for immunohistology on frozen unfixed sections of human, bovine and dog skin, of foetal bovine cornea, and of new dog tissue associated with a biomaterial implant. If fixation of tissue is required, acetone or ethanol is recommended. CSI 006-01 binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well. NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL.

COL5A1 encodes an alpha chain for one of the lowabundance fibrillar collagens. Fibrillar collagen molecules aretrimers that can be composed of one or more types of alpha chains.Type V collagen is found in tissues containing type I collagen andappears to regulate the assembly of heterotypic fibers composed ofboth type I and type V collagen. COL5A1 product is closelyrelated to type XI collagen and it is possible that the collagenchains of types V and XI constitute a single collagen type withtissue-specific chain combinations. Mutations in this gene areassociated with Ehlers-Danlos syndrome, types I and II.

Specifications

• Antigen: Collagen V (soluble)
• Applications: ELISA, Immunohistochemistry (Frozen), Western Blot
• Classification: Monoclonal
• Clone: 1E2-E4/Col5
• Concentration: 1.05 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 15mM sodium azide; pH 7.4
• Gene: COL5A1
• Gene Accession No.: P20908
• Gene Alias: AB collagen; AI413331; alpha-1 type V collagen; alpha1(V) collagen; COL5; Col5a1; Collagen 5; collagen alpha 1 (V); collagen alpha-1(V) chain; collagen type 5 alpha 1; collagen type V alpha 1; collagen type V alpha 1 chain; collagen, type V, alpha 1; EDSC; pro-alpha1(V) collagen; procollagen alpha 1(V); procollagen type V alpha 1; procollagen, type V, alpha 1; RP11-263F14.1; Type V Collagen
• Gene Symbols: COL5A1
• Host Species: Mouse
• Immunogen: Acid-digested pepsin soluble dog type V collagen.
• Purification Method: Protein A/G
• Quantity: 400 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 100327268, 100848491, 101107777, 1289, 397533, 480684, 537387
• Target Species: Human, Canine, Rabbit, Bovine, Sheep, Pig, Marsupial
• Content And Storage: 4°C, store in dark
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a κ