Invitrogen™ Complement C3b Monoclonal Antibody (11-10)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA544636

Catalog No. PIMA544636

Description

Complement C3b Monoclonal Antibody for Western Blot, ICC/IF, Flow

Inactive complement component 3b (iC3b) is a 42kDa neoantigen present in blood serum. Cleavage of C3b into iC3b by the protease factor I, in the presence of cofactors, changes the structure and resulting binding properties, preventing binding by complement factor B and properdin. This prevents further progression of the complement pathway, providing another mechanism by which the complement pathway may be regulated. iC3b is thought to play a role in inducing tolerance, binding complement receptor type 3 on antigen presenting cells and stimulating them to produce transforming growth factor beta2 and interleukin-10. It is thought to stimulate B-cells via the CD21 receptor providing a link between the innate and adaptive immune responses. Additionally iC3b acts as an opsonin which is recognised by complement receptor 3 expressed on leukocytes or complement receptor of the immunoglobulin superfamily (CRIg) on Kupffer cells, stimulating phagocytosis of the tagged cells. iC3b levels are often elevated in diseases such as Systemic Lupus Erythematosis and Rheumatoid arthritis.

Specifications

• Antigen: Complement C3b
• Classification: Monoclonal
• Concentration: 2 mg/mL
• Formulation: PBS with 0.2% BSA, 40% glycerol and 0.05% sodium azide; pH 7.4
• Gene Accession No.: P01024
• Gene Symbols: C3
• Immunogen: Synthetic peptide within human Complement C3(beta chain) 480-560.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Flow Cytometry, Western Blot, Immunocytochemistry
• Clone: 11-10
• Conjugate: Unconjugated
• Gene: C3
• Gene Alias: Acylation stimulating protein; acylation-stimulating protein cleavage product; AHUS5; AI255234; ARMD9; ASP; C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a; C3a anaphylatoxin; C3adesArg; C3b; C3bc; C3-beta-c; Complement C3; Complement C3 alpha chain; Complement C3 beta chain; Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3c alpha' chain fragment 2; Complement C3d fragment; Complement C3dg fragment; Complement C3f fragment; Complement C3g fragment; complement component 3; complement component C3a; complement component C3b; CPAMD1; ENCF-1; ENCF-2; epididymis secretory sperm binding protein Li 62p; HEL-S-62p; HSE-MSF; iC3b; Neutrophil chemotactic factor-1; Neutrophil chemotactic factor-2; Plp; prepro-C3
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 718
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid