Invitrogen™ Complement C9 Monoclonal Antibody (22)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ ABS0042202

Catalog No. ABS0042202

Description

ABS 004-22-02 is specific for complement factor C9. ABS 004-22-02 has been successfully used in ELISA and Western blot procedures. ABS 004-22-02 recognizes C9 in human serum diluted 1:50 in Tris buffer (20 mM Tris-base, 1 mM MgCl2, 1 mM CaCl2 and 140 mM NaCl) and incubated for 2 hours at 37°C using a human IgM coated (10 μg/mL overnight at 4°C, blocked with PBS 7.2 + 1% BSA for 1 hour) ELISA plate. NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL.

C5b-9 membrane attack complexes are assembled from five precursor molecules in the serum. Proteolytic cleavage of C5 by C5 convertase generates C5b which initiates assembly of the C5b-9 complex. The last step of C5b-9 complex formation involves polymerization of C9 which accompanies insertion of the complex into the cell membrane. During formation of C5b-8 and C9 polymerization, neoantigens are generated which are unique to the C5b-9 complex and are not present on any of the individual native complex components. The complement regulatory proteins CD59 and complement S-protein can both prevent C5b-9 insertion into the cell membrane. The formed SC5b-9 complex is unable to attach to cells and is cytolytically inactive. C5b-9 is involved in the progression of chronic proteinuric renal disease by mediating continuous tubulointerstitial damage. Early tubulointerstitial injury in the remnant kidney can be improved when C5b-9 complex forming is abrogated.

Specifications

• Antigen: Complement C9
• Classification: Monoclonal
• Concentration: 1.03 mg/mL
• Formulation: PBS with 15mM sodium azide; pH 7.4
• Gene Accession No.: P02748
• Gene Symbols: C9
• Immunogen: Complement component C9.
• Quantity: 400 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot
• Clone: 22
• Conjugate: Unconjugated
• Gene: C9
• Gene Alias: ARMD15; C9; C9D; complement C9; complement component 9; complement component C9; Complement component C9a; Complement component C9b; complement protein C9; complement protein C9 precursor
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 735
• Content And Storage: 4°C, store in dark
• Form: Liquid