Invitrogen™ Complement C9 Monoclonal Antibody (2A1)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA533355

Catalog No. PIMA533355

Description

Product is supplied at 1 mL (100 μL/mL). This antibody was shown to compete with antibodies to human C9 for its binding site on the C5b-9 complex, indicating that the reactive epitope is located on the C9 molecule. This product can be used as a coating antibody to detect C5b-9 in plasma and urine samples. Application Notes: ELISA - plates were coated with 30 μg/mL in 50mM carbonatebuffer, pH10.6 for 16h at 4°C. Western Blot - A non-reduced sample treatment and SDS-Page was used. The band size is >200 kDa. IHC-P - Tissue sections fixed in formalin were pretreated with protease type XXIV for 10 minutes at 37°C before incubation. IHC-F- Tissue sections were fixed in acetone for 10 minutes at room temperature before incubation.

C5b-9 membrane attack complexes are assembled from five precursor molecules in the serum. Proteolytic cleavage of C5 by C5 convertase generates C5b which initiates assembly of the C5b-9 complex. The last step of C5b-9 complex formation involves polymerization of C9 which accompanies insertion of the complex into the cell membrane. During formation of C5b-8 and C9 polymerization, neoantigens are generated which are unique to the C5b-9 complex and are not present on any of the individual native complex components. The complement regulatory proteins CD59 and complement S-protein can both prevent C5b-9 insertion into the cell membrane. The formed SC5b-9 complex is unable to attach to cells and is cytolytically inactive. C5b-9 is involved in the progression of chronic proteinuric renal disease by mediating continuous tubulointerstitial damage. Early tubulointerstitial injury in the remnant kidney can be improved when C5b-9 complex forming is abrogated.

Specifications

• Antigen: Complement C9
• Classification: Monoclonal
• Concentration: 100 μg/mL
• Formulation: PBS with 0.1% BSA and 0.02% sodium azide
• Gene Accession No.: Q62930
• Gene Symbols: C9
• Immunogen: Rat C5b-9.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 2A1
• Conjugate: Unconjugated
• Gene: C9
• Gene Alias: ARMD15; C9; C9D; complement C9; complement component 9; complement component C9; Complement component C9a; Complement component C9b; complement protein C9; complement protein C9 precursor
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 117512
• Content And Storage: 4°C
• Form: Liquid