Invitrogen™ Complement C9 Monoclonal Antibody (aE11)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA533373

Catalog No. PIMA533373

Description

Product is supplied at 1 mL (100 μL/mL). This antibody reacts with a C9 neoantigen of the terminal complement complex (TCC). Application Notes: Functional Assay - This antibody inhibits platelet activation by antiphospholipid antibody serum. IF - Cryosections of 10 μm were dried, fixed in acetone at 4°C for 10 min, dried and blocked for 20 min with PBSA. Positive control: Mucosa from patients with H. Pylori.

C5b-9 membrane attack complexes are assembled from five precursor molecules in the serum. Proteolytic cleavage of C5 by C5 convertase generates C5b which initiates assembly of the C5b-9 complex. The last step of C5b-9 complex formation involves polymerization of C9 which accompanies insertion of the complex into the cell membrane. During formation of C5b-8 and C9 polymerization, neoantigens are generated which are unique to the C5b-9 complex and are not present on any of the individual native complex components. The complement regulatory proteins CD59 and complement S-protein can both prevent C5b-9 insertion into the cell membrane. The formed SC5b-9 complex is unable to attach to cells and is cytolytically inactive. C5b-9 is involved in the progression of chronic proteinuric renal disease by mediating continuous tubulointerstitial damage. Early tubulointerstitial injury in the remnant kidney can be improved when C5b-9 complex forming is abrogated.

Specifications

• Antigen: Complement C9
• Classification: Monoclonal
• Concentration: 100 μg/mL
• Formulation: PBS with 0.1% BSA and no preservative
• Gene Accession No.: P02748, P48770
• Gene Symbols: C9
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 100034183, 100037951, 735
• Content And Storage: 4°C
• Form: Liquid

• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunocytochemistry, Western Blot
• Clone: aE11
• Conjugate: Unconjugated
• Gene: C9
• Gene Alias: ARMD15; C9; C9D; complement C9; complement component 9; complement component C9; Complement component C9a; Complement component C9b; complement protein C9; complement protein C9 precursor
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Pig, Horse
• Product Type: Antibody
• Isotype: IgG2a

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov