Invitrogen™ MTCO2 Monoclonal Antibody (4B12A5)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 459150

Catalog No. 45-915-0

Description

Positive control: Mitochondria from yeast membrane extract.

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix. [UniProt].

Specifications

• Antigen: MTCO2
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: HEPES buffered saline with 0.02% sodium azide
• Gene Accession No.: P00410
• Gene Symbols: COX2
• Immunogen: Full length purified native protein.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Yeast
• Product Type: Antibody
• Isotype: IgG2a κ

• Applications: Western Blot
• Clone: 4B12A5
• Conjugate: Unconjugated
• Gene: COX2
• Gene Alias: ACI60_gp02; Co2; COII; COX2; COXII; cyclooxygenase; cytchrome c oxidase sububnit 2; Cytochrome c oxidase polypeptide II; Cytochrome c oxidase subunit 2; cytochrome c oxidase subunit II; mitochondrially encoded cytochrome c oxidase II; Mtco2; MT-CO2; OXI1; OXII; Q0250
• Host Species: Mouse
• Purification Method: IgG fraction
• Regulatory Status: RUO
• Gene ID (Entrez): 24573139, 854622
• Content And Storage: 4°C, do not freeze
• Form: Liquid