Invitrogen™ COMT Monoclonal Antibody (15C10)

Catalog No.	Quantity
PIMA549153	100 μg

Catalog No. PIMA549153 Supplier Invitrogen™ Supplier No. MA549153

Description

Mouse Monoclonal Antibody

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Human COMT shares 81.9% and 81% amino acid (aa) sequence identity with mouse and rat COMT, respectively. Positive Control - WB: human HepG2 whole cell, human K562 whole cell, human THP-1 whole cell, human HEK293 whole cell, human A549 whole cell, human Caco-2 whole cell, rat RH35 whole cell, mouse Neuro-2a whole cell. IHC: human intestinal cancer tissue, human mammary cancer tissue, human mammary cancer tissue, human placenta tissue, human lung cancer tissue, mouse testis tissue, rat testis tissue. ICC/IF: A431 cell, MCF7 cell. Flow: K562 cell, U87 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.

Specifications

• Antigen: COMT
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: 15C10
• Concentration: 500 μg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 4mg trehalose and 0.05mg sodium azide
• Gene: Comt
• Gene Accession No.: O88587, P21964, P22734
• Gene Alias: Catechol O-methyltransferase; catecholamine-O-methyltransferase; catechol-O-methyltransferase; catechol-O-methyltransferase 1; catechol-O-methyltransferase isoform; Comt; Comt1; D16Wsu103e; D330014B15Rik; epididymis secretory sperm binding protein Li 98n; HEL-S-98n; OTTHUMP00000197749; OTTHUMP00000197750; testicular tissue protein Li 42
• Gene Symbols: Comt
• Host Species: Mouse
• Immunogen: E.coli-derived human COMT recombinant protein (Position: G52-P271).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 12846, 1312, 24267
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG2b