Invitrogen™ COMT Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA595276

Catalog No. PIPA595276

Description

Human COMT shares 81.9% and 81% amino acid (aa) sequence identity with mouse and rat COMT, respectively. Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: human K562 whole cell, human SH-SY5Y whole cell, human HepG2 whole cell, human A431 whole cell, human MCF-7 whole cell, rat liver tissue. IHC: Mouse Lung tissue, Rat Lung tissue, Human Placenta tissue IHC-F: human placenta tissue, mouse lung tissue, rat lung tissue. ICC/IF: A549 Cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.

Specifications

• Antigen: COMT
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Comt
• Gene Alias: Catechol O-methyltransferase; catecholamine-O-methyltransferase; catechol-O-methyltransferase; catechol-O-methyltransferase 1; catechol-O-methyltransferase isoform; Comt; Comt1; D16Wsu103e; D330014B15Rik; epididymis secretory sperm binding protein Li 98n; HEL-S-98n; OTTHUMP00000197749; OTTHUMP00000197750; testicular tissue protein Li 42
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12846, 1312, 24267
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 500 μg/mL
• Formulation: PBS with 5mg BSA and 0.05mg sodium azide
• Gene Accession No.: O88587, P21964, P22734
• Gene Symbols: Comt
• Immunogen: E.coli-derived human COMT recombinant protein (Position: G52-P271).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG