Invitrogen™ Connexin 43 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA532778

Catalog No. PIPA532778

Description

Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Hamster, Elephant, Panda, Rabbit, Pig, Opossum, Guinea pig (100%); Bat, Horse, Chicken, Lizard, Xenopus (94%); Trout, Salmon (88%); Zebrafish, Sea anemone, Nematode (81%). For IHC(P), use heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication. GFAP are membrane-spanning proteins that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is also targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. Mutations in the GFAP gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy, oculodentodigital dysplasia and heart malformations. Alternatively spliced transcript variants of GFAP have been found.

Specifications

• Antigen: Connexin 43
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: GJA1
• Gene Alias: alpha 1 connexin; AU042049; AVSD3; AW546267; CMDR; Cnx43; connexin 43; connexin43; connexin-43; cx43; Cx-43; cx43 protein; cx43a1; Cx43alpha1; CXA1; Cxn-43; DFNB38; EKVP; etID309742.9; gap junction 43 kDa heart protein; Gap junction alpha-1 protein; gap junction membrane channel protein; gap junction membrane channel protein alpha 1; gap junction protein; gap junction protein alpha 1; gap junction protein connexin 43; gap junction protein, alpha 1; gap junction protein, alpha 1, 43 kD (connexin 43); gap junction protein, alpha 1, 43kDa; gap junction protein, alpha 1, 43kDa (connexin 43); gja1; Gja-1; GJAL; HLHS1; HSS; Npm1; ODD; ODDD; ODOD; PPKCA; SDTY3; shf; Short fin protein; sof; stopsel; stp; Syndactyly type III; vascular smooth muscle connexin43; Vascular smooth muscle connexin-43; zfCx43.3
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100008935, 100379273, 100518636, 100689077, 14609, 24392, 2697, 281193, 403418
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin)
• Concentration: 1 mg/mL
• Formulation: PBS with 0.1% sodium azide
• Gene Accession No.: P08050, P17302, P18246, P23242, Q29101, Q6S9C0, Q6TYA7
• Gene Symbols: GJA1
• Immunogen: Synthetic 16 amino acid peptide from C-terminus of human GJA1 / Connexin 43.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Canine, Rabbit, Monkey, Bovine, Hamster, Pig, Guinea Pig
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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