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Eppendorf™ Centrifuge 5427 R

The refrigerated Centrifuge 5427 R was designed for high-throughput research applications. Featuring an aerosol-tight 48-place rotor and equipped with the QuickLock lid for easy rotor lid opening and closing, it is the safe, convenient, and reliable option.

Supplier:  Eppendorf™ 5409000136

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Catalog No. 05-100-151

Item Discontinued This item has been discontinued by the manufacturer and is no longer available. Please reach out to your sales representative. View substitute products »



  • Rotor capacity: 48 x 1.5/2.0 mL, 12 x 5.0 mL; Eppendorf QuickLock™ rotor allows for fast and ergonomic lid locking
  • Max. speed: 25,001 x g (16,220 rpm)
  • High capacity 48-place rotor significantly increases productivity to save you valuable time
  • Soft touch one-finger closure for ergonomic operation
  • Temperature range from -11°C to 40°C
  • FastTemp function for quick pre-cooling of the centrifuge, e.g., from ~ 23°C to 4°C in only 11 minutes
  • ECO shut-off can be programmed to engage after 1, 2, 4 or 8 hours of non-use for maximum energy savings and extended compressor life
  • Built-in condensation drain to eliminate water accumulation
  • Automatic rotor recognition and imbalance detection for maximum operational safety


30 x 2 mL
25,001 x g
10 in.
<46 dB(A) with Rotor F-45-48-11
12.6 in.
-11°C to +40°C
5427R Centrifuge
Lid Lock, steel armoured safety chamber, view port for speed validation, lid emergency release, certified aerosol-tight rotor lids
319 x 540 x 254 mm
550 W
66.1 lb. (30 kg)
230 V
16,220 rpm
Large LCD
25.4 cm
10 sec. to 9:59 h
31.9 cm
UL (120 V), CAN/CSA (120 V), CE, RoHS, IVD for certain catalog numbers
Without rotor
230 V, 50/60 Hz (EU)
Centrifuge 5427 R, rotary knobs
EN ISO 12100, EN 50581, EN 61010-1, EN 61010-2-020, IEC 61010-1, IEC 61010-2-020, EN 61326-1, EN 55011, EN 378-1, EN 378-2, EN 50581 (refer to manual for more details)
50/60 Hz


Product Certifications


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The Centrifuge 5427 R is a non-automatic centrifuge for the separation of liquid substance mixtures from the human body and is specifically intended to be used as an accessory together with an in vitro diagnostic device and to enable its use according to its intended purpose. This product complies with the requirements of the EU In Vitro Diagnostics Regulation (IVDR) 2017/746. Eppendorf centrifuges are exclusively intended for indoor use by trained specialists.