Invitrogen™ Histone Macro-H2A.1 Recombinant Rabbit Monoclonal Antibody (RM248)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA524696

Catalog No. PIMA524696

Description

This antibody reacts to the Core histone macro-H2A.1 (Histone macroH2A1) protein, independent of post-translational modifications. No cross reactivity with other histone proteins. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

Specifications

• Antigen: Histone Macro-H2A.1
• Classification: Recombinant Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA, 50% glycerol and 0.09% sodium azide; pH 7.2-7.4
• Gene Accession No.: O75367, Q9QZQ8
• Gene Symbols: MACROH2A1
• Immunogen: Peptide corresponding to the C-terminus of human Histone macroH2A1.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: ChIP Assay, ELISA, Western Blot, Luminex, Immunocytochemistry
• Clone: RM248
• Conjugate: Unconjugated
• Gene: MACROH2A1
• Gene Alias: Core histone macro-H2A.1; H2A histone family member Y; H2A histone family, member Y; H2A.y; H2A/y; H2AF12M; H2AFY; Histone H2A.y; histone macroH2A1; histone macroH2A1.1; histone macroH2A1.2; macroH2A1; MACROH2A1.1; macroH2A1.2; medulloblastoma antigen MU-MB-50.205; mH2A1
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 26914, 9555
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid