Invitrogen™ COX2 Polyclonal Antibody

Catalog No.	Quantity
PIPA588606	100 μL

Catalog No. PIPA588606 Supplier Invitrogen™ Supplier No. PA588606

Description

Rabbit Polyclonal Antibody

Positive Samples: A-549, HeLa, Mouse spleen, Mouse lung, Rat lung, Rat kidney; Cellular Location: Endoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein Immunogen sequence: PLLPDTFQIH DQKYNYQQFI YNNSILLEHG ITQFVESFTR QIAGRVAGGR NVPPAVQKVS QASIDQSRQM KYQSFNEYRK RFMLKPYESF EELTGEKEMS AELEALYGDI DAVELYPALL VEKPRPDAIF GETMVEVGAP FSLKGLMGNV ICSPAYWKPS TFGGEVGFQI INTASIQSLI CNNVKGCPFT SFSVPDPELI KTVTINASSS RSGLDDINPT VLLKERSTEL.

COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins. The conversion of arachidonate to prostaglandin H2 is a 2 step reaction: a cyclooxygenase (COX) reaction which converts arachidonate to prostaglandin G2 (PGG2) and a peroxidase reaction in which PGG2 is reduced to prostaglandin H2 (PGH2). It is constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and is up-regulated under pathological conditions, such as in cancer and inflammation (in contrast to the iso-enzyme PTGS1, which is expressed ubiquitously). Up-regulation of COX2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, COX2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. COX2 is naturally inhibited by calcitriol (the active form of Vitamin D). Glucocorticoids chronically trans-repress PTGS2 gene activity in vivo in part by interfering with transcription initiation and elongation. COX2 is a target of NSAID such as aspirin, which can reduce pain and swelling from inflammation driven by COX2.

Specifications

• Antigen: COX2
• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot, Western Blot
• Classification: Polyclonal
• Concentration: 0.57 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 0.05% ProClin 300; pH 7.3
• Gene: PTGS2
• Gene Accession No.: P35354, P35355, Q05769
• Gene Alias: COII; COX2; Cox-2; COXII; cyclooxygenase; cyclooxygenase 2; cyclooxygenase 2b; cyclooxygenase-2; Cytochrome c oxidase polypeptide II; Cytochrome c oxidase subunit 2; cytochrome c oxidase subunit II; glucocorticoid-regulated inflammatory cyclooxygenase; Gripghs; hCox-2; Macrophage activation-associated marker protein P71/73; mitochondrially encoded cytochrome c oxidase II; MTCO2; MT-CO2; PES-2; PGG/HS; PGH synthase 2; Pghs2; PGHS-2; Pghs-b; PHS II; PHS-2; prostaglandin G/H synthase 2; Prostaglandin H2 synthase 2; prostaglandin-endoperoxide synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); Ptgs2; Tis10; TIS10 protein
• Gene Symbols: PTGS2
• Host Species: Rabbit
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 375-604 of COX2/PTGS2 (NP_0009541).
• Purification Method: Affinity Chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 19225, 29527, 5743
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG