Invitrogen™ COX5B Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA596189

Catalog No. PIPA596189

Description

Immunogen sequence: ASGGGVPTDE EQATGLEREI MLAAKKGLDP YNVLAPKGAS GTREDPNLVP SISNKRIVGC ICEEDNTSVV WFWLHKGEAQ RCPRCGAHYK LVPQQLAH; Positive Samples: HepG2, 22Rv1, SW480, HeLa, A-549, Mouse heart, Mouse kidney, Rat heart; Cellular Location: Mitochondrion inner membrane.

The cytochrome c oxidase (COX) family of proteins function as the final electron donor in the respiratory chain to drive a proton gradient across the inner mitochondrial membrane, ultimately resulting in the production of water. The mammalian COX apoenzyme is a dimer, with each monomer consisting of 13 subunits, some of which are mitochondrial and some of which are nuclear. Found in the inner mitochondrial membrane, COX5 is the heme A-containing chain of the oxidase family that converts one molecule of oxygen and four molecules of hydrogen to two molecules of water. Two isoforms of COX5 exist, COX5a and COX5b. When oxygen levels within the cell are high, transcription of COX5a (the aerobic isoform) is up-regulated as the rate of cellular respiration increases. Conversely, when oxygen levels are low, COX5b (the hypoxic isoform) transcription increases and functions to maximize the turnover rate of the COX apoenzyme.

Specifications

• Antigen: COX5B
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: COX5B
• Gene Alias: COX5B; COXVB; cytochrome c oxidase polypeptide Vb; cytochrome c oxidase polypeptide VB, mitochondrial; cytochrome c oxidase polypeptide VIa; cytochrome c oxidase precursor (EC 1.9.3.1); cytochrome c oxidase subunit 5B; cytochrome c oxidase subunit 5B, mitochondrial; cytochrome c oxidase subunit Vb; cytochrome c oxidase subunit VIA*
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12859, 1329, 94194
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 1.68 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: P10606, P12075, P19536
• Gene Symbols: COX5B
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 32-129 of human COX5B (NP_0018532).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG