Invitrogen™ COX5B Recombinant Rabbit Monoclonal Antibody (020)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA529172

Catalog No. PIMA529172

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. This antibody has specificity for Human COX5B.

The cytochrome c oxidase (COX) family of proteins function as the final electron donor in the respiratory chain to drive a proton gradient across the inner mitochondrial membrane, ultimately resulting in the production of water. The mammalian COX apoenzyme is a dimer, with each monomer consisting of 13 subunits, some of which are mitochondrial and some of which are nuclear. Found in the inner mitochondrial membrane, COX5 is the heme A-containing chain of the oxidase family that converts one molecule of oxygen and four molecules of hydrogen to two molecules of water. Two isoforms of COX5 exist, COX5a and COX5b. When oxygen levels within the cell are high, transcription of COX5a (the aerobic isoform) is up-regulated as the rate of cellular respiration increases. Conversely, when oxygen levels are low, COX5b (the hypoxic isoform) transcription increases and functions to maximize the turnover rate of the COX apoenzyme.

Specifications

• Antigen: COX5B
• Classification: Recombinant Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P10606
• Gene Symbols: COX5B
• Immunogen: Recombinant Human COX5B Protein (Ala32-His129).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: ELISA, Immunoprecipitation, Western Blot
• Clone: 020
• Conjugate: Unconjugated
• Gene: COX5B
• Gene Alias: COX5B; COXVB; cytochrome c oxidase polypeptide Vb; cytochrome c oxidase polypeptide VB, mitochondrial; cytochrome c oxidase polypeptide VIa; cytochrome c oxidase precursor (EC 1.9.3.1); cytochrome c oxidase subunit 5B; cytochrome c oxidase subunit 5B, mitochondrial; cytochrome c oxidase subunit Vb; cytochrome c oxidase subunit VIA*
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 1329
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid