Invitrogen™ CPSF4 Polyclonal Antibody

Catalog No.	Quantity
PIPA5111614	100 μL

Catalog No. PIPA5111614 Supplier Invitrogen™ Supplier No. PA5111614

Description

Rabbit Polyclonal Antibody

Immunogen sequence: PSCKFMHPRF ELPMGTTEQP PLPQQTQPPA KQSNNPPLQR SSSLIQLTSQ NSSPNQQ Highest antigen sequence indentity to the following orthologs: Rat - 53%, Mouse - 98%.

Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene.

Specifications

• Antigen: CPSF4
• Applications: Immunocytochemistry
• Classification: Polyclonal
• Concentration: 0.2 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene: CPSF4
• Gene Accession No.: O95639
• Gene Alias: 30kDa; C79664; cleavage and polyadenylation specific factor 4; cleavage and polyadenylation specific factor 4, 30kD subunit; cleavage and polyadenylation specific factor 4, 30kDa; cleavage and polyadenylation specificity factor 30 kDa subunit; Cleavage and polyadenylation specificity factor subunit 4; clipper homolog; Clipper/CPSF 30K; CPSF 30 kDa subunit; Cpsf30; CPSF4; musculus clipper/cleavage and polyadenylation specificity factor 30 kDa subunit; NAR; NEB1; NEB-1; no; no arches homolog; no arches-like zinc finger protein; NS1 effector domain-binding protein 1
• Gene Symbols: CPSF4
• Host Species: Rabbit
• Immunogen: Recombinant Protein corresonding to Human CPSF4. Recombinant protein control fragment (Product #RP-107497).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 10898
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG