CRASP-1 Polyclonal Antibody, Rockland™

Catalog No.	Quantity
50-199-0900	100 μg
50-199-0901	25 μL

Catalog No. 50-199-0900 Supplier Rockland Immunochemicals Supplier No. 200401C18

Description

Rabbit Polyclonal Antibody

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This antibody is specific for Borrelia burgdorferi CRASP-1 protein. A BLAST analysis was used to suggest reactivity with CRASP-1 from B. burgdorferi and B. garinii sources based on 100% homology with the immunizing sequence. Partial cross-reactivity is expected against B. spielmanii, afzelii, and valaisiana sources based on 98% homology. Cross-reactivity with CRASP-1 from other sources has not been determined.

CRASP-1, or Complement Regulator-Acquiring Surface Protein 1, is a multifunctional protein of Lyme disease-causing B. burgdorferi that binds to several human extracellular matrix proteins and plasminogen, including factor H (resulting in inhibition of complement activation in mammals) and Human Bone Morphogenic Protein 2. These interactions may contribute to adhesion, bacterial colonization, and organ tropism and may allow dissemination of B. burgdorferi in the host. B. burgdorferi spirochetes express up to 5 complement regulator-acquiring surface proteins. Multiple copies of sequences analagous to CRASP-1 genes have been detected in Borrelia plasmids. Borrelia species contain a large number of plasmids, of linear and circular, some of which appear to repeat sequences or contain fragments of other genes. These regions may serve as potentially usable information for the survival of Borrelia in its multiple environments during its life cycle. In addition, the sequence for CRASP-1 contains a repeated sequence folded into a stable stem loop structure typical of RNA genes.

Specifications

• Antigen: CRASP-1
• Applications: ELISA, Immunoprecipitation, Western Blot
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Host Species: Rabbit
• Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi CRASP-1 protein.
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Bacteria
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Lyophilized