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CRISPR-Cas9 Mouse anti-Human, Clone: 1E8, Bioss
SDP

Catalog No. 501989540
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50-198-9540 100 μL
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Catalog No. 50-198-9540 Supplier Bioss Supplier No. BSM52042M
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Description

Mouse Monoclonal Antibody

Cas9 is a RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering. Staphylococcus aureus derived Cas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity. SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 (Cas9 derived from S. pyogenes) with an ideal spacer lengths of 24 to 20 nucleotides. In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM. This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.

Specifications

Antigen CRISPR-Cas9
Applications Western Blot
Classification Monoclonal
Clone 1E8
Concentration 1 μg/mL
Conjugate Unconjugated
Formulation TBS with 1% BSA, 40% glycerol and 0.05% sodium azide; pH
Gene CASP9
Gene Accession No. P55211
Gene Alias APAF3; APAF-3; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; cas9; CASP 9; CASP9; CASP-9; caspase 9; caspase 9, apoptosis-related cysteine peptidase; Caspase9; Caspase-9; Caspase-9 precursor; Caspase-9 subunit p10; Caspase-9 subunit p35; CASPASE-9c; CRISPR-Cas9; CTD; ICE-LAP6; ICE-like apoptotic protease 6; MCH6; OTTHUMP00000002324; PPP1R56; protein phosphatase 1, regulatory subunit 56; RNCASP9; RP11-265F14.3
Gene Symbols CASP9
Host Species Mouse
Immunogen Recombinant fragment corresponding to Streptococcus pyogenes CRISPR-Cas9 (100-200aa).
Purification Method Protein A
Quantity 100 μL
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 842
Target Species Human
Content And Storage -20°C
Product Type Antibody
Form Liquid
Isotype IgM
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