Invitrogen™ CrkL Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA595058

Catalog No. PIPA595058

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: human K562 whole cell, human 293T whole cell, human U20S whole cell, human Hela whole cell, rat thymus tissue, rat brain tissue, mouse thymus tissue, mouse brain tissue. Flow: U251 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

The CRKL gene encodes the CRK-like protein, an adaptor protein that plays a crucial role in intracellular signaling pathways related to cell proliferation, differentiation, and migration. CRKL contains SH2 and SH3 domains that facilitate interactions with other signaling molecules, influencing multiple pathways such as the MAPK, PI3K, and STAT pathways. It is involved in processes like cytoskeleton reorganization and immune cell signaling, with significant implications for development and cancer. CRKL is notably associated with Philadelphia chromosome-positive leukemias, where it interacts with the BCR-ABL fusion protein, contributing to leukemogenesis. Additionally, dysregulation of CRKL can affect cardiovascular development, leading to congenital heart defects. Research into CRKL focuses on understanding its role in signal transduction and its potential as a therapeutic target due to its involvement in oncogenic and developmental pathways.

Specifications

• Antigen: CrkL
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CRKL
• Gene Alias: 1110025F07Rik; AA589403; AI325100; CRK like proto-oncogene, adaptor protein; CRKL; crkl {ECO:0000312; crk-like protein; crk-like protein {ECO:0000250; Crkol; EMBL:AAH85865.1, ECO:0000312; OTTHUMP00000198539; RGD:1308531}; UniProtKB:P46109}; v-crk avian sarcoma virus CT10 oncogene homolog-like; v-crk sarcoma virus CT10 oncogene homolog (avian)-like; v-crk sarcoma virus CT10 oncogene homolog-like
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12929, 1399, 287942
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Flow Cytometry, Western Blot, Immunocytochemistry
• Concentration: 500 μg/mL
• Formulation: PBS with 5mg BSA and 0.05mg sodium azide, 0.05mg thimerosal
• Gene Accession No.: P46109, P47941, Q5U2U2
• Gene Symbols: CRKL
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CrkL (286-303aa FPFTHVKIFDPQNPDENE).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG