Invitrogen™ CRY1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA589349

Catalog No. PIPA589349

Description

Positive Samples: Mouse testis, Mouse eye, Rat testis, SW480; Cellular Location: Cytoplasm, Nucleus Immunogen sequence: GLGLLASVPS NPNGNGGFMG YSAENIPGCS SSGSCSQGSG ILHYAHGDSQ QTHLLKQGRS SMGTGLSGGK RPSQEEDTQS IGPKVQRQST N.

Various biochemical, physiological and behavioral processes display circadian rhythms controlled by an internal biological clock. The central gears driving this clock appear to be composed of an autoregulatory transcription/post translation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function; in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.

Specifications

• Antigen: CRY1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CRY1
• Gene Alias: AU020726; AU021000; Cry1; cryptochrome 1 (photolyase-like); cryptochrome circadian clock 1; cryptochrome-1; PHLL1
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12952, 1407, 299691
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1.92 mg/mL
• Formulation: PBS with 50% glycerol and 0.05% ProClin 300; pH 7.3
• Gene Accession No.: P97784, Q16526, Q32Q86
• Gene Symbols: CRY1
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 496-586 of human CRY1 (NP_0040661).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG