CRY2 Mouse anti-Human, Clone: OTI1H5, lyophilized, TrueMAB™

Catalog No.	Quantity
50-166-1722	100 μg

Catalog No. 50-166-1722 Supplier OriGene Supplier No. CF502905

Description

Mouse Monoclonal Antibody

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product No. 7KMWCO

Various biochemical, physiological and behavioural processes display circadian rhythms controlled by an internal biological clock. The central ""gears"" driving this clock appear to be composed of an autoregulatory transcription/posttranslation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function; in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.

Specifications

• Antigen: CRY2
• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: OTI1H5
• Conjugate: Unconjugated
• Formulation: PBS with 8% trehalose and no preservative; pH 7.3
• Gene: CRY2
• Gene Accession No.: Q49AN0
• Gene Alias: HCRY2, PHLL2
• Gene Symbols: CRY2
• Host Species: Mouse
• Immunogen: Full length human recombit protein of human CRY2 produced in HEK293T cell.
• Purification Method: Affinity Chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 1408
• Target Species: Human
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG2a