Invitrogen™ CRYAB Monoclonal Antibody (1A7.D5), PE

Catalog No.	Quantity
PIMA545201	200 μg

Catalog No. PIMA545201

Supplier: Invitrogen™ MA545201

Description

Mouse Monoclonal Antibody

0.5 μg/mL of MA5-45201 was sufficient for detection of 50 ng purified alpha B crystalline by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 20kDa (Predicted mol. weight is approximately 21kDa). Does not cross-react with alpha A-crystallin, beta L-crystallin, beta H-crystallin, gamma -crystallin, HSP25, HSP27 or HSP47 proteins.

Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products-alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.

Specifications

• Antigen: CRYAB
• Applications: ELISA, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: 1A7.D5
• Concentration: 1 mg/mL
• Conjugate: PE
• Formulation: 2.48mM MES, 95.64mM phosphate with 0.5M EDTA and no preservative; pH 7.2
• Gene: Cryab
• Gene Accession No.: P02510, P02511, P23928, Q7M2W6
• Gene Alias: 2310001E10Rik; 3526402H21Rik; AACRYA; active transcription factor CREB; alpha B-crystallin; alpha crystallin B; alpha(B)-crystallin; alpha-B-crystallin; Alpha-crystallin B chain; alpha-crystallin B chain-like protein; AV083133; cAMP response element binding protein 1; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CMD1II; CREB; CREB1; CREB-1; Crya2; Crya-2; CRYAB; CryAB antibody; crystallin alpha B; crystallin, alpha 2; crystallin, alpha B; crystallin, alpha polypeptide 2; CTPP2; CTRCT16; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; cyclic AMP-responsive element-binding protein 1; epididymis secretory protein Li 101; heat shock protein beta-5; heat shock protein CryAB; heat shock protein HspB5; heat-shock 20 kD like-protein; HEL-S-101; HSPB5; HspB5 protein; MFM2; P23; Renal carcinoma antigen NY-REN-27; rosenthal fiber component; transactivator protein; Y protein
• Gene Symbols: Cryab
• Host Species: Mouse
• Immunogen: Native Alpha B Crystallin.
• Purification Method: Protein G
• Quantity: 200 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 100519789, 1410, 25420, 281719
• Target Species: Human, Rat, Bovine, Pig
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1